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S9.6抗體Anti-DNA-RNA Hybrid [S9.6] Antibody

更新時(shí)間:2025-02-26

簡(jiǎn)要描述:

ThismousemonoclonalantibodywasgeneratedagainstaΦX174bacteriophage-derivedsyntheticDNA–RNAantigenandrecognizesRNA-DNAhybridsofvariouslengths

This mouse monoclonal antibody was generated against a ΦX174 bacteriophage-derived synthetic DNA–RNA antigen and recognizes RNA-DNA hybrids of various lengths.

Highlights:

- Useful in the detection of R-loops

- High specificity and affinity for DNA-RNA hybrids

- Does NOT cross-react with single-stranded DNA or double-stranded DNA

- Minor cross-reaction (~5-fold less) has been observed for AU-rich double-stranded RNA.

- High affinity binding shown for hybrids of 8, 10, 15, and 23 base pairs in length

DNA-RNA hybrids are a natural occurrence within eukaryotic cells, with levels of these hybrids increasing at sites with high transcriptional activity, such as during transcription initiation, repression, and elongation. Because RNA-DNA hybrids influence genomic instability, the S9.6 antibody is a useful reagent to help study the consequences of R-loops and lesions formed by these hybrids during DNA replication or other cellular processes. In addition, the S9.6 antibody is effective in recognizing RNA-DNA hybridization for microarray studies.


Product Type:Antibody
Name:Anti-DNA-RNA Hybrid [S9.6]
Antigen:S9.6 ΦX174 bacteriophage-derived synthetic DNA–RNA antigen
Isotype:Mouse IgG2a
Clonality:Monoclonal
Clone Name:S9.6
Reactivity:High specificity and affinity for DNA/RNA hybrids and other A-form nucleic acid hybrids
Immunogen:ΦX174 bacteriophage-derived synthetic DNA/RNA
Species Immunized:BALB/c mouse
Paratope:DYYGSRWFDY (proposed)
Purification Method:Protein A/G
Buffer:PBS, 0.05% (w/v) Sodium Azide
Tested Applications:Immunoblot/Immunoprecipitation,  Chip/Chip-seq, Immunocytochemistry, Immunofluorescence, DNA-RNA Immunoprecipitation (DRIP)
Storage:-20C (avoid repeated freeze-thaw cycles)
Shipped:Cold packs

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